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Table 4 Details on the sugarcane microsatellite loci derived from expressed sequence tags (ESTs)

From: A novel linkage map of sugarcane with evidence for clustering of retrotransposon-based markers

Marker code

Repeat motif

Forward primer (5′→3′)

Reverse primer (5′→3′)

PCRe

ATf

Dg

Allele number and size rangeh

ESTA26 a

(TG)11

GGCAGCCCCACATCTTCCT

GGGCACAAGCATCCGAACC

1

56.0

S

4

172-186

ESTA48 a

(CA)8

AGCAACTCCGGCCTCTCCTG

CTTTCTGTTTTGCTCCTCCGTCTG

1

62.7

S

10

233-295

ESTA53 a

(TG)8

TGGAAATGGCAGCTGGTCTCGT

ATGCACGTACCAGAGGGAGATTTG

1

58.9

S

9

168-192

ESTA61 a

(AT)12

ACCTCAGTCTCCTCCTCAACC

TATACTACACATGCACAGGCTACG

1

56.4

S

5

236-246

ESTB14 a

(CGT)8

TGAGGGAATGAATGGACTGG

CCACCACCACCATACCTGTC

1

52.0

S

9

285-315

ESTB55 a

(CCA)5

CTTCTTGGCCTTGGCGTTACTGA

GCTAGCTGGCCCCATTTCCTCT

1

60.0

S

3

118-124

ESTB60 a

(TTG)10

AGCCGCAATGAATCCAACTG

CTCTAGCTCCGACGATGATACCTC

1

61.0

S

8

157-206

ESTB82 a

(CGT)9

CGTCGATCGAGATGAAGAAGG

GAAGCAGTCGTGGAAGTGGAG

1

62.7

S

5

245-263

ESTB94 a

(CTT)9

GAGGCAGCCAGGCAGGTCAC

GGTGGCAGTGTTCAGGCAGATG

1

61.0

S

10

210-279

ESTB99 a

(TCG)5

GAGGTCCTTCTTGTAGTTGTATGC

GTGCCGGAGGATTTGATG

1

64.7

S

4

215-224

ESTB100 a

(TCG)6

CCACGGGCGAGGACGAGTA

GGGTCCTTCTTCGCCTCGTG

1

64.7

S

13

240-278

ESTB118 a

(TTC)6

CTTGGCTAGGGTTTCTTGAGTCGT

CATGGCTTTTGGCTTGCTTCT

1

61.0

S

5

106-163

ESTB189 b

(TCA)10

GTAAGGAAGAAGCAACAAACAACAG

GATTCGATGCAACTCTCCTGTAAA

1

60.0

S

5

261-280

ESTB191 b

(GCT)5

GCGCCATCAGGGAAGCCAAAAC

GCGCGTGCGAGCAGATGAAC

1

60.0

S

5

213-226

ESTC80 a

(ATTC)3

ATTCTTTCTCCCCCTGTTGTGC

GTCGCCAGATCGCTTTCGTT

1

58.9

S

7

188-292

CV06 c

(AATT)13

TCTCAAGCTTCGCCAGCTA

TGGCTCGGCTGTAGGAATTA

2

60.0

S

3

188-230

CV11 c

(GAA)6

TGGCATGTGTCATAGCCAAT

CCCCAACTGGGACTTTTACA

2

60.0

S

6

227-242

CV22 c

(AGGG)5

CACTACTCGCCCCGATTTC

CGAGTGCTTCTCCATCTGC

2

64.0

F

8

140-166

CV23 c

(GGAA)7/(AGG)6

GAACTGCTCACTGGCTCCTC

GTAGAAGTCCGTCGCCGTAA

2

64.0

F

9

150-206

CV24 c

(CCAA)5/(CACCT)4

TCGGAGAAGTTGACCGAGTT

GGTTTAGAGTTGGGGCCTTC

2

60.0

F

7

187-205

CV29 d

(ATCT)14

TCGCGTCCACCAATGTAACC

GCGTGCATCGCTTGTGTCTT

2

64.0

F

10

85-133

CV37 d

(TTTC)15

GGATGGACGACGTGTCCTGG

ATAAAGTGGCCGCTTGGATTGA

2

64.0

F

6

117-155

CV38 d

(CTTTT)18

GAAGCAGGGGCCTCAAGTTG

GTCAAACAGGCGATCTGGCTC

2

64.0

F

9

109-199

CV46 c

(GGTAA)11

TGTTCCAAGTTCATGCGCTCC

ATGCATGCAGGTTCAAAAGCAG

2

64.0

F

5

146-188

CV51 c

(TGT)13

CTACCCCAACTTGCTTGGGAC

GACTGGAACAAAGACGGACTG

2

64.0

F

3

147-160

CV53 c

(AAAAT)5/(TTTAT)6

CCCCACCGTAGCTTGTGCAT

AAACGTGCACATGCTTGTATGC

2

64.0

F

7

160-183

CV58 c

(ATAGAT)10

CGGGTAGTTAGGAGGAGATGG

GTCATCCATTTTGGAACGAATGG

2

64.0

F

6

153-195

CV78 c

(CTGTG)9

ACGAGGCCACCATAGAACATG

GCAATTGGGAGGAGAGGAATG

2

64.0

F

9

144-203

CV79 c

(CTATAT)11/(TATAGA)6

GGCACTGCTGGTGGTTGATTG

TCCCACATCAAGAGGCAGCTA

2

64.0

F

7

136-197

CV86 c

(AATT)8

CCTCAGCAGCCCAAAGTCCT

GTCGGAATCAGCCGGATTAGC

2

64.0

F

5

159-187

CV91 c

(GCC)6/(GCA)6

AAAGGAAATCGCCCTCCGTCT

CCGATGATGAGCCAGCAATCC

2

64.0

F

8

175-197

CV94 c

(AAAAAG)5/(CGT)5

GGCAGGCCAAGATGAATGAAG

AGCACAGCGGAGGGTACGG

2

64.0

F

4

187-205

CV100 c

(GAG)13

CTGTTGAGGAGCCGGATGAG

CTCTTCCGATGGCTCGGTCT

2

64.0

F

9

222-256

CV101 c

(ATC)23

GTCGTCGTCGTCACGATCATC

AGTTGACGGCATGGTTCTTGC

2

64.0

F

11

111-180

CV104 c

(TCCTG)5

GATTTTCGACTGTGCGCTTGG

AAGTTCTCTGCCGGAGCAAAC

2

64.0

F

6

133-158

CV106 c

(GGC)8

AAACAGAGCATACTCGAGGCC

ACGTTGCTGACGAGGTTTTCC

2

64.0

F

6

146-161

CV115 c

(TCACAG)10/(GTA)6/(AGA)5

GTCCATGTCCATCCATGATCC

GGAGCTCCGTCTTCTTGTTAC

2

60.0

S

6

150-174

CV119 c

(AAAAC)7

TATCTCTCCTTGGTTTGGATGG

CACCCTACCAAATACCACAACA

2

64.0

F

5

121-175

CV128 c

(GCA)13

AGGGCAACGGAGTCTTCGAC

CTGAACTCCGATGTGCTGGTG

2

60.0

F

5

147-168

CV135 c

(AAG)16

AGCAAAACCAGCCTTCCCTTC

CTGTTTGTTTCTGCTTGCTTGC

2

64.0

F

6

129-159

CV144 c

(TCTCCG)5

GCGCCTCCGTGGATAAGAATC

CCTTCCCCTACAGCGCCTAC

2

64.0

F

5

146-164

  1. a, b, c, d Develop by Oliveira et al.[72], Marconi et al.[73], CanaVialis (unpublished); Maccheroni et al.[74], respectively.
  2. e PCR program as described in Material and Methods.
  3. f Annealing temperature in the amplification reaction.
  4. g D: Silver-stained polyacrylamide gel electrophoresis (S) or fluorescence-based automated capillary electrophoresis (F) for the detection of EST-SSR alleles.
  5. h Observed number of alleles per locus and their size ranges in bp.