Figure 4

Mutation rates of mcm2 alleles and strains with gene deletions that suppress mcm2_AA. The mutation rates of MCM2 and mcm2_AA strains with and without gene deletions that suppress mcm2_AA were calculated as described [39, 40] using the CAN1 forward mutation assay. Dark grey bars are the deletions in the MCM2 background; white bars are with mcm2_AA and the light grey bar (far left) is the mcm2_EE strain. The error bars represent the upper and lower confidence limits (95%) of the mutation rates and were calculated from the 95% confidence limits of the median determined from the binomial distribution [40].