Figure 1

Giemsa-stained karyotypes of Aplastodiscus. A. A. perviridis , male, 2n = 24; B. A. arildae, male, 2n = 22; C. A. eugenioi, male, 2n = 22; D. A. callipygius, male, 2n = 20. E. A. leucopygius, male, 2n = 18. Insets show marker pairs, visualised by Ag-NOR and FISH with the rDNA probe. Bar = 10 μm.