Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions

Table 5 Protein structural predictions for the three apple LRRs based on their primary sequences

Apple cultivar	LRR allele	Signal peptide¹	Location²	Predicted stability (ΔΔG)³
‘M.26’, ‘M.27’, R5-US, R5-NZ	MxdRLP1-1	1-24	Outside cell	NA
R5-US, ‘G.41’, R5-NZ, AJ103	MxdRLP1-2	1-24	Outside cell	−0.95 ⁴; -0.05 ⁵ Kcal/mol
‘G.41’	MxdRLP1-3	1-24	Outside cell	−1.98 Kcal/mol

¹Predictions were based on analysis by PSORT [70], PSPIRED [71] and Polyphobius [72].
²Prediction was based on PSORT [70] and Polyphobius [72].
³Predictions were based on analysis by I-Mutant2.0 [78]. Standard error for predictions based on protein sequence is 1.45.
⁴Allele MxdRLP1-2 using only the Gly to Ala substitution was used to perform prediction analysis, since the I-Mutant2.0 program will only analyze one amino acid substitution at a time from a single polypeptide.
⁵Allele MxdRLP1-2 using only the Leu to Phe substitution was used to perform prediction analysis.

ISSN: 2730-6844