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Table 4 Alleles of the Malus leucine-rich repeat family receptor-like protein gene ( MxdRLP1 )

From: Putative resistance gene markers associated with quantitative trait loci for fire blight resistance in Malus ‘Robusta 5’ accessions

Base Pair1

EST consensus2

Mxd RLP1-1

Mxd RLP1-2

Mxd RLP1-3

Mxd RLP1-4

Mxd RLP1-5

126

A

A

G

A

A

A

135

T

T

T

C

T

T

221

A

A

A

T*

A

A

405

C

C

A

C

C

C

438

C

C

C

C

C

A

451

G

G

G

G

A*

G

456

C

C

A

C

C

C

465

A

A

T

A

A

A

611

G

G

C*

G

G

G

645

G

G

T*

G

G

G

651

C

C

C

C

T

C

756

G

G

G

G

G

T

897

G

C

C

C

C

C

969

A

A

G

G

A

A

1053

G

G

G

G

G

A

1062

C

T

T

T

T

C

Found in3:

M.26 (1)

AJ103 (5)

G.41 (3)

M.27 (3)

M.9 (10)

  

M.27 (7)

G.41 (3)

  

AJ103 (3)

  

R5-DE (3)

R5-DE (5)

   
  

R5-NZ (8)

R5-NZ (4)

   
  

R5-US (4)

R5-US (3)

   
  1. Single nucleotide polymorphisms (SNPs, in bold type) detected in five alleles of putative fire blight resistance gene MxdRLP1 that was identified from EST GenBank:EB140229, co-locates with the fire blight resistance QTL on LG3 and is homologous to Arabidopsis thaliana leucine-rich repeat family protein gene At3g20820 (Genebank:NP188718). Asterisk (*) indicates SNPs resulting in amino acid substitutions.
  2. 1Base pair numbered from adenine of presumed ATG start codon of GenBank accessions JN798165-798172.
  3. 2Consensus sequence resulting from alignment of 1) Genome Database for the Rosaceae Malus EST assembly version 4 contig17331 [53], 2) TIGR Malus x domestica transcript assembly release 2 contig TA23938_3750 [54], and 3) the PFR bioinformatics database [43] sequence for MxdRLP1. The GDR and TIGR contigs were selected based upon BLASTN comparison with EST EB140229. ESTs within consensus sequence were derived from several different cultivars.
  4. 3 Alleles of MxdRLP1 were identified from sequence alignment of multiple DNA clones obtained from fire blight resistant AJ103 (M9xR5-NZ progeny), ‘Geneva 41’ (G.41), ‘Robusta5’-DE (R5-DE), ‘Robusta 5’-NZ (R5-NZ), and ‘Robusta 5’-US (R5-US), and susceptible ‘Malling 9’ (M.9), ‘Malling 26’ (M.26), and ‘Malling 27’ (M.7). Number of DNA clones obtained for each allele is in parentheses following cultivar name.