Base Pair1 | EST consensus2 | Mxd RLP1-1 | Mxd RLP1-2 | Mxd RLP1-3 | Mxd RLP1-4 | Mxd RLP1-5 |
---|
126 | A | A |
G
| A | A | A |
135 | T | T | T |
C
| T | T |
221 | A | A | A | T* | A | A |
405 | C | C |
A
| C | C | C |
438 | C | C | C | C | C |
A
|
451 | G | G | G | G | A* | G |
456 | C | C |
A
| C | C | C |
465 | A | A |
T
| A | A | A |
611 | G | G |
C*
| G | G | G |
645 | G | G |
T*
| G | G | G |
651 | C | C | C | C |
T
| C |
756 | G | G | G | G | G |
T
|
897 |
G
| C | C | C | C | C |
969 | A | A |
G
|
G
| A | A |
1053 | G | G | G | G | G | A |
1062 |
C
| T | T | T | T |
C
|
Found in3: | M.26 (1) | AJ103 (5) | G.41 (3) | M.27 (3) | M.9 (10) |
| | M.27 (7) | G.41 (3) | | | AJ103 (3) |
| | R5-DE (3) | R5-DE (5) | | | |
| | R5-NZ (8) | R5-NZ (4) | | | |
| | R5-US (4) | R5-US (3) | | | |
- Single nucleotide polymorphisms (SNPs, in bold type) detected in five alleles of putative fire blight resistance gene MxdRLP1 that was identified from EST GenBank:EB140229, co-locates with the fire blight resistance QTL on LG3 and is homologous to Arabidopsis thaliana leucine-rich repeat family protein gene At3g20820 (Genebank:NP188718). Asterisk (*) indicates SNPs resulting in amino acid substitutions.
- 1Base pair numbered from adenine of presumed ATG start codon of GenBank accessions JN798165-798172.
- 2Consensus sequence resulting from alignment of 1) Genome Database for the Rosaceae Malus EST assembly version 4 contig17331 [53], 2) TIGR Malus x domestica transcript assembly release 2 contig TA23938_3750 [54], and 3) the PFR bioinformatics database [43] sequence for MxdRLP1. The GDR and TIGR contigs were selected based upon BLASTN comparison with EST EB140229. ESTs within consensus sequence were derived from several different cultivars.
- 3 Alleles of MxdRLP1 were identified from sequence alignment of multiple DNA clones obtained from fire blight resistant AJ103 (M9xR5-NZ progeny), ‘Geneva 41’ (G.41), ‘Robusta5’-DE (R5-DE), ‘Robusta 5’-NZ (R5-NZ), and ‘Robusta 5’-US (R5-US), and susceptible ‘Malling 9’ (M.9), ‘Malling 26’ (M.26), and ‘Malling 27’ (M.7). Number of DNA clones obtained for each allele is in parentheses following cultivar name.