Figure 4From: Loss of nonsense mediated decay suppresses mutations in Saccharomyces cerevisiae TRA1Phenotypes of TRA1-F3744A suppressed by upf1Δ. Serial dilutions of yeast strains CY4353 (TRA1 UPF1), CY4350 (tra1-F3744A UPF1), CY5932 (TRA1 upf1Δ), and CY6030 (tra1-F3744A upf1Δ) haploids were spotted onto YPD, YP plus 2% galactose, YPD depleted of phosphate, or YPD containing 1 μg/mL phleomycin, 10 μg/mL Calcofluor white, or 2 nM rapamycin.Back to article page