MTH1 and RGT1 demonstrate combined haploinsufficiency in regulation of the hexose transporter genes in Saccharomyces cerevisiae

Table 2 β-galactosidase activity of HXT 2, 3 and 4 promoter fusions to LacZ

Relevant Genotype	LacZ Fusion Reporter Assay^*
	β-Galactosidase Activity (nmole/min/mg protein)
	*HXT2*	*HXT3*	*HXT4*
Wild Type	323.8±7.4a	41.5±3.7b	31.4±1.5c
snf3/snf3	2.8±0.07e	1.3±0.07e	1.0±0.37e
snf3/snf3 rgt1/RGT1 mth1/MTH1	26.6±1.8d	2.8±0.2e	1.6±0.04e

^*Strains were grown to mid log phase in SC-URA + 2% galactose and then transferred to SC-URA with 0.05% glucose plus Antimycin A and harvested and assayed 3 hours after the shift. Values represent the average +/− standard deviation of at least three separate transformants cultured separately with the same reporter construct. Different letters denote significant differences at p <0.05.

ISSN: 2730-6844