Table 4 PCR primer sequence for candidate QTL-related gene and SNP type used for genotypic analysis
Candidate genes | Primer sequence (5'→3') | SNP |
|---|---|---|
Lp EUI | f: ACG TAC CTG TAC TGG CTG | C/G |
| Â | r: TTG CAG TTG TCC ACC ACG AA | Â |
Lp PDT | f: GCA GAA CAA AAA CTC CAA GA | n.a. *a |
| Â | r: TTG GAT CAG CCA TAG ACG CC | Â |
Lp GA3ox2 | f: CGC GCT ACT TCG ACT TCC | n.a. *b |
| Â | r: GAA GAA GGA GGA GAT GGG C | Â |
Lp DGL1 | f: GTT AAC ATT GAT GAA GTT GC | A/G |
| Â | r: ACA CTC TTC TGG ACC TTG GC | Â |
Lp GA20 | f: GGG TGT ACC AGG AGT ACT G | n.a. *a |
| Â | r: TTA CCA TGA AGG TGT CGC CG | Â |
Lp Ph1 | f: GCA TTA ATG ATG AAT GGG CT | A/G |
| Â | r: CAT CCA CAC CAG TTA TTC TC | Â |
Lp Pi37 | f: CCA GCG GAT ATG CGC AAT CT | C/T |
| Â | r: CAA ATG CTC TCG GCT GAA GG | Â |
Lp PIPK1 | f: GGC CCT TGT AAA TAG TCT CC | G/T |
| Â | r: CCC TTG ACT GTA ATT GGC TC | Â |
Lp Pib | f: TCA CGG ATG AGA TCA TGG AC | A/T |
| Â | r: CTG AAG AAG TGT GAT GGA CT | Â |
Lp D3 | f: CCA AGA TGA AAT TGG ACC TC | A/G |
| Â | r: CTG CAT GTC CCG CAA GTT TG | Â |
*a no SNP was identified in PCR amplicon. | Â | |
*b PCR amplicons were not obtained. | Â | |