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Table 1 Strains used in this study

From: Functional characterization of the Saccharomyces cerevisiae protein Chl1 reveals the role of sister chromatid cohesion in the maintenance of spindle length during S-phase arrest

Strain

Genotype

Sources/References

AP22

MAT α leu2-3,112 his3-11,15 ura3-52 trp1

[36]

AP22Dchl1

MAT α leu2-3,112 his3-11,15 ura3-52 trp1 chl1::HIS3

[36]

8534-8C

MAT α his4Δ34 ura3-52 leu2-3,112

[69]

8534-10A

MAT a his4Δ34 ura3-52 leu2-3,112

[73]

301-2B

MATα leu2-3,112 hisΔ34 ura3-52 trp1

[66]

PS29-2B

MAT a leu2-3,112 his3-11,15 mcm18-1/ctf19

[69, 74]a

M29-5D

MAT a leu2-3,112 hisΔ34 ura3-52 trp1mcm18-1/ctf19

This study, by crossing 301-2B with PS29-2B

A3

MAT a leu2-3,112 his3-11,15

[69]

699

MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100

[37]

699Matα

MATα ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100

This study

699Dchl1

MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 chl1::HIS3

[37]

SL14

MAT a ade2-1 trp1-1 leu2-3, 112 his 3-11, 15 ura3 can1-100 bar1Δ::LEU2

[37]

SL14Dchl1

MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3 can1-100 bar1Δ::LEU2 chl1::HIS3

[37]

US354

MATα leu2 his3 trp1 ade2 ura3 rad53-21

[37]

SL7

MAT a leu2 his3 trp1 ade2 ura3 rad53-21

[37]

US3329

MAT a leu2::LEU2::tetR-GFP trp1 CEN5::tetOX224::HIS3 ade2-1 ura3 his3

[75]

US3324

MAT a ade2-1 leu2-3,112 his 3-11,15 can1-100 scc1-73

Uttam Surana

SL20

MATα ade2-1 can1-100 leu2-3,112 his3-11,15 scc1-73

This study, by crossing US3324 with 699Matα

SL25

MAT a leu2::LEU2 tetR-GFP ade2-1 CEN5::tetOX224::HIS3 ura3 scc1-73

This study, by crossing US3329 with SL20

US3329Δchl1

MAT a leu2::LEU2:: tetR-GFP ura3 CEN5::tetO X224::HIS3 ade2-1 chl1Δ::TRP1

This study, by deleting CHL1 in US3329

US3329Δmcm17

MAT a leu2::LEU2:: tetR-GFP ura3 CEN5::tetOX224::HIS3 ade2-1 mcm17Δ::URA3

This study, by deleting MCM17 in US3329

US3329Dmcm21

MAT a leu2::LEU2:: tetR-GFP ura3 CEN5::tetOX224::HIS3 ade2 mcm21::URA3

This study, by disrupting MCM21 in US3329

US3335

MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1

Uttam Surana

US3335Dchl1

MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 chl1::HIS3

This study, by disrupting CHL1 in US3335

US3335Δsir3

MAT a ade2-1 trp1-1 leu2-3,112 his 3-11,15 ura3-1 can1-100 SCC1-18MYC::TRP1 sir3Δ::HIS3

This study, by deleting SIR3 in US3335

  1. 699 and all the strains listed below it are in W303 background, while the strains listed above 699 were from G. Fink.
  2. a The MCM18 gene is the same as CTF19 [74, Saccharomyces Genome Database http://www.yeastgenome.org]. The PS29-2B strain [69] contains the mcm18-1 allele of the MCM18/CTF19 gene.
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BMC Genomic Data

ISSN: 2730-6844

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