Figure 4

Normalised expression of SCARB1 and SCARB1-2 transcripts in Atlantic salmon tissues. Real-time PCR analyses showing the normalised SCARB1 and SCARB1-2 mRNA levels in Atlantic salmon muscle, liver and mid gut (n = 7-10), calculated by the 2^-ΔΔCt method and adjusted for PCR efficiencies. When comparing the gene expression in mid gut, muscle and liver for each paralog using one-way ANOVA, SCARB1 was found to be significantly increased in midgut compared to muscle and liver (P < 0.01). Average values of three independent qPCR runs were used for the calculation.