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Table 2 Primers for SNP detection in the porcine APOE 5'-regulatory region

From: A functional mutation at position -155 in porcine APOE promoter affects gene expression

Primers

Sequences

Annealing temperature

PCR product length

P1

Upstream: 5'-TCGAGAGGGAGTGAGGGTTAAAA-3'

59°C

538 bp

 

Downstream: 5'-AGGAGCAGGACCAGAAGG-3'

  

P2

Upstream: 5'-GGCCTTCTGGTCCTGCTCCT-3'

61°C

455 bp

 

Downstream: 5'-AACTCCTGCCTCGGTGGTGG-3'

  

P3

Upstream: 5'-CACCGAGGCAGGAGTTGG-3'

60°C

728 bp

 

Downstream: 5'-CTTCTGGCTTGCGATTGG-3'

  
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BMC Genomic Data

ISSN: 2730-6844

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