Reverse-transcriptase (RT)-PCR of NESP55 expression in foetal and adult tissues. RT-PCR primers were located in the NESP55 exon (forward primer) and GNAS exon 6 (reverse primer). Lanes 1-8: RT-PCR product of tissues from 8 weeks old foetus; lane 9 and 19: RT-PCR product from liver from the dam of 8 and 10 weeks old foetus, respectively; lanes 10-18: RT-PCR product of tissues from 10 week old foetus. Amplified fragments were confirmed by sequencing to be NESP55 transcript. C - PCR negative control; M - HyperLadder II Marker (Bioline Ltd, London, UK).