From: Genetic effects of polymorphisms in candidate genes and the QTL region on chicken age at first egg
No. | Sites1 | Chr2 | Gene | Primer sequence (5'→3') | Length3 (bp) | AT4(°C) | Restriction enzyme |
---|---|---|---|---|---|---|---|
M1 | G840327C | Chr22 | GnRH-I | F:tgtcacacccaggatctcaa R:gctgttcagaggcacgtgag | 310 | 59.0 | MnII |
M2 | C31394761T | Chr2 | NPY | F:cgtggctgctttgcttcctttc R:ggggtacgaggcaaggacatg | 324 | 60.0 | Kpn I |
M3 | T5841629C | Chr24 | DRD2 | F:tgcacataaaagcccactcactg R:gcctgagctggtgggggg | 248 | 60.0 | BseGI |
M4 | G51389822T | Chr3 | VIP | F:gcttggactgatgcgtactt R:gtatcactgcaaatgctctgc | 520 | 58.0 | Apo I |
M5 | A1661691G | Chr2 | VIPR-1 | F:tgaaagcccccaggatct R:agcaaaacaaaacccaaatca | 364 | 58.2 | Tai I |
M6 | C1704887T | Chr2 | VIPR-1 | F:ccccgttaaactcagcagac R:cccaaagtcccacaaggtaa | 434 | 58.2 | Hha I |
M7 | C1715301T | Chr2 | VIPR-1 | F:ctcctcaggcagaccatcatg R:cttgcacgtatccttgggtagc | 486 | 58.2 | Taq I |
M8 | I59724210D | Chr2 | PRL | F:tttaatattggtgggtgaagagaca R:atgccactgatcctcgaaaactc | 130/154 | 54.0 | PCR |