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Table 1 Primers of mutations in the candidate genes

From: Genetic effects of polymorphisms in candidate genes and the QTL region on chicken age at first egg

No.

Sites1

Chr2

Gene

Primer sequence

(5'→3')

Length3 (bp)

AT4(°C)

Restriction enzyme

M1

G840327C

Chr22

GnRH-I

F:tgtcacacccaggatctcaa

R:gctgttcagaggcacgtgag

310

59.0

MnII

M2

C31394761T

Chr2

NPY

F:cgtggctgctttgcttcctttc

R:ggggtacgaggcaaggacatg

324

60.0

Kpn I

M3

T5841629C

Chr24

DRD2

F:tgcacataaaagcccactcactg

R:gcctgagctggtgggggg

248

60.0

BseGI

M4

G51389822T

Chr3

VIP

F:gcttggactgatgcgtactt

R:gtatcactgcaaatgctctgc

520

58.0

Apo I

M5

A1661691G

Chr2

VIPR-1

F:tgaaagcccccaggatct

R:agcaaaacaaaacccaaatca

364

58.2

Tai I

M6

C1704887T

Chr2

VIPR-1

F:ccccgttaaactcagcagac

R:cccaaagtcccacaaggtaa

434

58.2

Hha I

M7

C1715301T

Chr2

VIPR-1

F:ctcctcaggcagaccatcatg

R:cttgcacgtatccttgggtagc

486

58.2

Taq I

M8

I59724210D

Chr2

PRL

F:tttaatattggtgggtgaagagaca

R:atgccactgatcctcgaaaactc

130/154

54.0

PCR

  1. 1The sites were based on the chicken genome sequences released in May, 2006 http://genome.ucsc.edu/cgi-bin/hgGateway;
  2. 2Chr referred to chromosome;
  3. 3Length indicated the length of PCR products;
  4. 4AT referred to annealing temperature.