A major QTL controls susceptibility to spinal curvature in the curveback guppy

BMC Genomic Data

Table 1 The distribution of curve magnitude among curveback mapping offspring

	Qualitative value for curve magnitude									Total offspring	Total curved	Percent curved
	0	1		2		3		4
Backcross	67	32	52%	23	37%	5	8%	2	3%	129	62	48%
F2 - Cross 1	224	40	64%	16	26%	6	10%	0	n/a	286	62	22%
F2 - Cross 2	200	11	19%	35	59%	10	17%	3	5%	259	59	29%

Backcross offspring are from Cross 1. Fish were scored for curve magnitude using a qualitative scale [7]: 0 = non-curved to 4 = extreme curve. Mapping offspring are euthanized at a minimum of 3 months past birth (sexual maturity is at about 1 month past birth), photographed, and frozen in buffered (EDTA) ethanol. The same person scored each individual alive, and then scores were confirmed in photos. Among each phenotypic class (score), the proportion of fish among curved individuals is shown. All fish were photographed on a light table with a digital camera under 3X magnification.

ISSN: 2730-6844