Figure 2
From: Gene cassette transcription in a large integron-associated array
A flow chart of the molecular methods used in this work. Parallel extractions of gDNA and RNA from a single cell culture with subsequent reverse transcription of the RNA, allowed an analytical comparison to be made between the gDNA and cDNA gene cassette profiles by an attC specific PCR. The RNA sample acted as a control for gDNA contamination present in the derived cDNA sample.