Table 1 PCR Primers
From: Co-assortment in integron-associated gene cassette assemblages in environmental DNA samples
PCR PRIMER | SEQUENCE 5' > 3' | COMMENTS |
|---|---|---|
HS527/HS286 | 6-FAM, GGGATCCTC(S)GCT(K)GARCGA(M)TTGTTAG(V)C | attC fwd |
HS528/HS287 | 6-FAM, GGGATCCGC(S)GCT(K)ANCTC(V)(R)(R)CGTTAG(S)C | attC rev |
MRG 53b | GATGGCGTCGCGAGTCAACC | 258bp fwd |
MRG 54 | GCCTTGCAATCCGTCAGCCACG | 258bp rev |
MRG 55 | AGTCAGACCTCAGGAACAAGAGC | 347 bp fwd |
MRG 56 | CAGCCTTTGCTCTTACTGCGAACC | 347 bp rev |
MRG57a | CTGCTTCCATTGTAAGAACACC | 429 bp fwd |
MRG58a | TCGGCACAAGTACAGTCTATGC | 429 bp rev |
MRG59a | TCTACGATCGCTATGGCAACGA | 482 bp fwd |
MRG60a | TGAGCCAATATTGAGGCAAGCA | 482 bp rev |
16SF | AGAGTTTGATCMTTGGCTCAG | 16S fwd |
16SR | TACGGYTACCTTGTTACGACTT | 16S rev |