Skip to main content

Table 1 PCR, sequencing, site-directed mutagenesis primer and TaqMan probe Sequences

From: Little ROCK is a ROCK1 pseudogene expressed in human smooth muscle cells

Assay Name Sequence
PCR or Sequencing LTEX3F 5'-TTTTAAAGAATCTAAGTCCTAAGCG-3'
  LTEX3R 5'-TACACATAAGTTAGTTCATTGAGAC-3'
  LTEX4F 5'-CATCAGCAAGAGATATGCTGC-3'
  LTEX4R 5'-CCTCTGTGGTGAAAAGCACAA-3'
  cDNALTF 5'-TCTCTGCACAGATCACCCAGTAAACTG-3'
  cDNARKF 5'-GCTGGAAGAAACAGTATGTTGTGG-3'
  cDNACMR 5'-TAGCATCCCACACGATTCCAC-3'
  5RACELT 5'-CTTTGGCACAGGCATCACAATTGGC-3'
  ROCKF 5'-ATGGTACGATGTGATACAGCG-3'
  ROCKR 5'-CTCACTTCCCTGTCAGTAAGG-3'
  GAPDHF 5'-GTCGGAGTCAACGGATTTGGT-3'
  GAPDHR 5'-GCCATGGGTGGAATCATATTGG-3'
  LTGFPF 5'-CCGGAATTCGAACCAGTACAAGAAGCTGAA-3
TaqMan Assay Rs1045144F 5'-TCCACCATCTGGTTTTGTTCGT-3'
  Rs1045144R 5'-CGGAAAGACTGATTTGCAGTGGAT-3'
  rs1045144-VIC 5'-TTCCCCTTGAACGCT-3'
  rs1045144-FAM 5'-CCCCTCGAACGCT-3'
  rs1045142/rs2663698F 5'-CCAAACCTCTCTGGCATGTTTT-3'
  rs1045142/rs2663698R 5'-CTTTCTTATCTAAGTGATCTCTGTGGCA-3'
  rs1045142/rs2663698-VIC 5'-CATGGCATCTTCGACACT-3'
  rs1045142/rs2663698-FAM 5'-ATGGCGTCTTTGACACT-3'
Mutagenesis MU2LTR 5'-ACTTAACATGGCATCTTCGACACTCTAGGG-3'
  MU2LTF 5'-CCCTAGAGTGTCGAAGATGCCATGTTAAGT-3'