Table 1 PCR, sequencing, site-directed mutagenesis primer and TaqMan probe Sequences
From: Little ROCK is a ROCK1 pseudogene expressed in human smooth muscle cells
Assay | Name | Sequence |
|---|---|---|
PCR or Sequencing | LTEX3F | 5'-TTTTAAAGAATCTAAGTCCTAAGCG-3' |
| Â | LTEX3R | 5'-TACACATAAGTTAGTTCATTGAGAC-3' |
| Â | LTEX4F | 5'-CATCAGCAAGAGATATGCTGC-3' |
| Â | LTEX4R | 5'-CCTCTGTGGTGAAAAGCACAA-3' |
| Â | cDNALTF | 5'-TCTCTGCACAGATCACCCAGTAAACTG-3' |
| Â | cDNARKF | 5'-GCTGGAAGAAACAGTATGTTGTGG-3' |
| Â | cDNACMR | 5'-TAGCATCCCACACGATTCCAC-3' |
| Â | 5RACELT | 5'-CTTTGGCACAGGCATCACAATTGGC-3' |
| Â | ROCKF | 5'-ATGGTACGATGTGATACAGCG-3' |
| Â | ROCKR | 5'-CTCACTTCCCTGTCAGTAAGG-3' |
| Â | GAPDHF | 5'-GTCGGAGTCAACGGATTTGGT-3' |
| Â | GAPDHR | 5'-GCCATGGGTGGAATCATATTGG-3' |
| Â | LTGFPF | 5'-CCGGAATTCGAACCAGTACAAGAAGCTGAA-3 |
TaqMan Assay | Rs1045144F | 5'-TCCACCATCTGGTTTTGTTCGT-3' |
| Â | Rs1045144R | 5'-CGGAAAGACTGATTTGCAGTGGAT-3' |
| Â | rs1045144-VIC | 5'-TTCCCCTTGAACGCT-3' |
| Â | rs1045144-FAM | 5'-CCCCTCGAACGCT-3' |
| Â | rs1045142/rs2663698F | 5'-CCAAACCTCTCTGGCATGTTTT-3' |
| Â | rs1045142/rs2663698R | 5'-CTTTCTTATCTAAGTGATCTCTGTGGCA-3' |
| Â | rs1045142/rs2663698-VIC | 5'-CATGGCATCTTCGACACT-3' |
| Â | rs1045142/rs2663698-FAM | 5'-ATGGCGTCTTTGACACT-3' |
Mutagenesis | MU2LTR | 5'-ACTTAACATGGCATCTTCGACACTCTAGGG-3' |
| Â | MU2LTF | 5'-CCCTAGAGTGTCGAAGATGCCATGTTAAGT-3' |