Little ROCK1 Peptide is Unstable. (A) Western blot of transfected HeLa cells probed with an antibody to GFP. Non-transfected control cell lysates are shown in lanes 1 and 2. Transfected cells were treated with MG132 (+) or control solvent (-). Expression of EGFP (lanes 3 and 4) was not affected by MG132 treatment. Cells expressing EGFP-LR-GT (lanes 5 and 6) and EGFP-LR-AC (lanes 7 and 8) included a slower migrating band consistent with the EGFP-Little Rock fusion peptide. MG132 treatment increased the abundance of the EGFP-Little ROCK fusion peptides. (B) Bar graph demonstrates mean ± SD intensity of peptide band normalized to treatment with solvent control. The experiment was performed three times; asterisk indicates the mean intensity of MG132-treated recombinant protein level was significantly different (p < 0.05) versus control treatment as determined by t-test analysis.