Little ROCK Expression Analysis. (A) Nucleotide sequence of a clone isolated by 5'RACE confirmed the Little ROCK transcript includes splicing of sequences with high identity to ROCK1 exon 30 to the Little ROCK-specific Exon 1. Arrowheads indicate exon splice sites. (B) Little ROCK, ROCK1 and GAPDH amplicons from cDNA produced from Lanes: 1-Testis, 2-Heart, 3-Kidney, 4-Brain, 5- Liver, 6- Lung, 7-Pancreas, 8-Placenta, and 9-Sk. Muscle. Lanes 10 to 16 are from cultured human VSMC lines: 10-IM230, 11-Ao146, 12-IM306, 13-Ao297, 14-IM337, 15-Co338, 16-Ao207. Lane 17 shows a negative control. GAPDH was used as a cDNA loading control.