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Table 2 Detail information for primers of the chicken DRD1 gene

From: The genetic effects of the dopamine D1 receptor gene on chicken egg production and broodiness traits

Primer name

Primer sequence

(5'→3')

Length1 (bp)

Location2

AT3 (°C)

Genotyping method

P1

F:CCGGTGAGTACCCTGCTTT

R:GTGCTTTTCCTCTGCTTTGG

1504

-1961 ~ -458

59

/

P2

F:AGTGAAGAATTGCTCGCTGA

R:GGTTTTGCTGGGTACACCTT

1970

-589 ~ +1381

57

/

P3

F:CACTATGGATGGGGAAGGGTTG

R: GGCCACCCAGATGTTGCAAAATG

283

G+123A

T+198C

62

BseNI

cfrI

P4

F: CAGCCCATTCAGGTACGAGAGGA

R: ATTCGACTCTTTGGGGCTGGAC

793

A+505G

C+765T

C+1011T

G+1065A

C+1107T

65.5

sequencing

P5

F: TTTCCTTCATCCCCGTGCAGCT

R: GCTGCTTCTGTTGCCACTTGTGT

290

+458 ~ +747

63

/

P6 (actin)

F: CCCCAAAGCCAACAGAGAGA

R: GGTGGTGAAGCTGTAGCCTCTC

274

/

63

/

  1. 1The length of PCR products; 2referred to the locations in the DRD1 gene, the first nucleotide of translation start codon was designated as +1. 3indicated annealing temperature.