Table 1 Primers and parameters used in the PCR
Gene | Sequences of the primers | Denaturation | Annealing | Elongation |
|---|---|---|---|---|
28S | LR10R: 5'GACCCTGTTGAGCTTGA3' LR12: 5'GACTTAGAGGCGTTCAG3' | 95°C, 20 s | 48°C, 20 s | 72°C, 30 s |
5S | F: 5'CAACGTGATATGGTCGTAGAC3' R: 5'AACACCGGTTCTCGTCCGATC3' | 95°C, 30 s | 44°C, 30 s | 72°C, 30 s |
h2ba | F: 5'TCCCGAGATGTGATGGTAGA3' R: 5'AGTACAGCCTGGATGTTTGGTAA3' | 95°C, 30 s | 45°C, 30 s | 72°C, 40 s |
h3 | F: 5'ATGGCTCGTACCAAGCAGACVGC3' R: 5'ATATCCTTRGGCATRATRGTGAC3' | 95°C, 15 s | 48°C, 15 s | 72°C, 15 s |