Figure 5

Immunolocalization of Yem-alpha and centromeric CID on oocyte whole mounts and cryo-sections. Whole mounts (Panels A and B) and cryosections (panel C) were observed by epifluorescence. To the exception of the pictures of panel (A), all other pictures were deconvolved. A - The panel represents staining of whole mount ovary for DNA (DAPI), Yem-alpha [14, 23] and Orb [52]. Yem-alpha is specifically localized to the oocyte nucleus whereas Orb specifically marks the ooplasm [14, 52]. FC: follicle cells NC: nurse cells; OO: ooplasm; ON: oocyte nucleus; st: oocyte stage. The arrow points out the oocyte and the arrowhead points out the oocyte nucleus. B - Gallery of whole mount egg chambers of various stages stained as indicated in the Methods section with anti Yem-alpha [14, 23] and anti-CID antibody [30]. Yem-alpha is localized within the whole nucleoplasm with the highest intensity colocalizing with the CID signal that specifically marks the centromeric region. The arrowhead points out the oocyte nucleus (ON). C - The panel displays a 40× magnification of a transverse section through the meiotic spindle of a mature oocyte (left), a 160× magnification of the same nucleus (middle) and zooms on 3 representative structures that are numbered 1, 2, 3 (right). These pictures were deconvolved. Yem-alpha staining was performed using a rabbit polyclonal antiserum [14, 23]. CID was revealed with a chicken anti-CID antibody [30]. DNA was stained with DAPI.