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Table 3 Primers used for amplification and sequencing the ovine BCMO1 and BCO2 genes

From: A nonsense mutation in the beta-carotene oxygenase 2 (BCO2) gene is tightly associated with accumulation of carotenoids in adipose tissue in sheep (Ovis aries)

Name

Direction

Position

Sequence5'-3'

BCMO1 - primers

F0025

Forward

-109 to -90a

CATCTGAAGGGAGGGAGATG

F0066

Forward

-85 to -66b

AAGACAAGGAGTGGCCAAGA

F0157

Forward

46-65b

GTGAGGGCCARAGTRACAGG

R0673

Reverse

520-539b

TTTCCAGCAGCATCGTAGTG

F1226

Forward

1092-1111b

CGTGGACAAGAATGCAGAAG

R1464

Reverse

1334-1352b

CTCCTCTCTCCACGTCAAGG

R1976

Reverse

1826-1845a

GATAGTCCTCACGGCCAAAA

BCO2 - primers

7541

Forward

-25 to -6c

CTGCTGCTGCAGAACTCAAC

7542

Reverse

648-666d

ATGTGCAGTTGCTCCGTTC

7543

Forward

591-611d

GGACATTGAAACCCTGGAAAA

7544

Reverse

1392-1404d

CCATTGAATTGGCCATAGTTG

7545

Forward

1287-1306d

TTCAGCCAGTGCTGTGAAAC

7546

Reverse

1758-1777c

AGCGAGCACATTCATTCAAA

7547

Forward

-52 to -34c

AGCGTGAGGATTTGGGAAT

7548

Reverse

588-607d

CCAGGGTTTCAATGTCCACT

7550

Forward

104-126d

TGGAACAGACTCATCAGAAAACA

7551

Reverse

257-276d

GAATTTCCCAGGTCCAACAC

BCO2F

Forward

170-189d

TTCTAACCACGGTGGAAGAG

BCO2R

Reverse

230-249d

ATAGCCATTGAGCCACTCAG

BCO2E

Forward

177-195d

CACGGTGGAAGAGACTCTG

  1. a. Positions are numbered according to first base of the translation start codon of the bovine BCMO1 gene (NM_001024559.1).
  2. b. Positions are numbered according to first base of the translation start codon of the ovine BCMO1 gene (FN543098)
  3. c. Positions are numbered according to first base of the translation start codon of the bovine BCO2 gene (NM_001101987).
  4. d. Positions are numbered according to first base of the translation start codon of the ovine BCO2 gene (FN257486).