Global alignment of 10-kb sequences around each NBS gene in the REN1 interval. (A) Comparison between the NBS founders of the REN1 interval (NBSc, NBSg, NBSj, NBSk). (B) Comparison between NBS gene pairs that have evolved from a duplication of a common ancestral sequence within the REN1 interval, based on the widest sharing of the highest nucleotide identity. The conserved blocks of nucleotide sequences and the corresponding level of identity were calculated using LAGAN and drawn using VISTA. The coding regions corresponding to the coiled coil (orange), NBS (green), and LRR (yellow) domains are indicated by coloured bars. The NBS domain was defined as the portion comprised from the P-loop to the MHDV motif. The insertion of repetitive elements is indicated by pink bars with the identifier of the gene in which the insertion has occurred. DNA sequences are represented from left to right, according to the top-to-bottom orientation of the assembled genome sequence along chr13. All protein coding sequences, with the exception of NBSg, are represented in an N- to C-terminus orientation from right to left.