Saturation of the REN1 interval in 'Kishmish vatkana' and links to the PN40024 sequence assembly. (A) Reference linkage map of chr13, as reported in . (B) Local linkage map in 'Kishmish vatkana' around the former REN1 interval (light grey segment) comprised between markers VMC3D12-VVIC51 and VVIP10 as previously defined by . The former REN1 region (dark grey segment) as identified by the heterozygous markers available in  was delimited by markers (bold) UDV124 on the proximal side and VMC9H4-2 and VMC4E10-1 on the distal side of chr13. (C) Sequence scaffolds in the PN40024 genome assembly. Markers already present on the previous version of the 'Kishmish vatkana' map  were projected onto the scaffolds. Scaffolds whose relative positions were known in PN40024 and for which markers were lacking in the 'Kishmish vatkana' map were used for developing targeted markers that saturate the REN1 interval.