Hypermethylation of the DLC1 CpG island does not alter gene expression in canine lymphoma

BMC Genomic Data

Table 2 Results of COBRA and MSP methylation analysis and real time expression analysis

Dog	B or T Cell	Rel Intensity Meth at HpyCh4IV	Rel Intensity Meth at TaqA	Rel Intensity Meth at BstuI	Meth:Unmeth Ratio for MSP	Relative Expression
C1	Normal	0.0461	0	0	0	0.3618
C2	Normal	0	0	0	0	N/A
C3	Normal	0	0	0	0	0.0103
C4	Normal	0	0	0	0	N/A
C5	Normal	0	0	0	0	N/A
C6	Normal	0.142	0.1148	0	0	0.0175
C7	Normal	0.1234	0.0573	0	0	0.0248
1	B	0	1	0	0.2666	0.0686
2	T	0.1961	1	0	0.0661	0.0992
3	B	0	1	0.2625	0	0.0752
4	B	0.1501	0.7505	0	0.9251	0.0164
5	B	0.1616	0.6651	0.4209	0.4932	0.1984
6	B	0.1821	0.7968	0	0.2628	0.0034
7	B	0.1407	0.1093	0.1042	0.3039	0.0260
8	B	0.1345	0.4357	0.2742	0.3431	0.2078
9	B	0	1	0.2481	0.4366	0.1575
10	B	0.1794	1	0.3558	0.1679	0.0655
11	B	0	0.2191	0	0.5008	0.2127
12	B	0	1	0	0.17993	0.0412
13	B	0	1	0	0	0.0884
14	T	0.1428	0.0655	0	0	0.0508
15	B	0	0	0	0.3781	0.0171
16	B	0.0351	0.4653	0	0.0614	0.1063
17	B	0	1	0	0.1700	3.0314
18	B	0.116	1	0	0.1543	0.1895
19	B	0.1654	1	0.4372	0.1366	0.2806
20	B	0	1	0	0	0.1403
21	B	0	0	0	0.1355	0.0000
Totals		11/21	19/21	7/21	17/21
P value for lymphoma		1.00	0.009	0.141	<0.001

There are seven control and 21 lymphoma samples. COBRA results are described as relative intensity of the methylated band out of all bands in the lane. The MSP results are described as a ratio between the methylated and unmethylated primer sets. Because these primer sets use different conditions for amplification, the ratio cannot be directly interpreted as a proportion of methylated DNA, but as a number relative to the other samples. Samples marked N/A were unavailable for the particular analysis.

ISSN: 2730-6844