Composite representation of original gels for COBRA and MSP results. Control animals are listed C1-C7. Cases are listed 1-21. Positive methylation controls are in the far right lane. Molecular makers are in the far left lane. Depicted are the COBRA results with digestion using BstuI, TaqaI, and HpyCh4IV. Methylation specific PCR results are depicted in the fourth row for the methylated primers and the fifth row for the unmethylated primers. All faint bands in the initial gels were confirmed in repeated assays.