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Table 1 Diversity of alternatively spliced transcripts of Bruno-3 identified in four different species of Drosophila

From: Large introns in relation to alternative splicing and gene evolution: a case study of Drosophila bruno-3

   Size of translated exon (bp) Number of sampled clones
AST Size of ORF 2 3 4 4a 5 6 7 8 9 10 11 12 13 14 D. mel. (19) D. pse. (51) D. per. (11) D. vir. (19)
1 1281 60 - 49 - 135 - 80 57 144 274 99 78 177 128 N/A 1   N/A
2, A 1269 60 42 49 - 135 - 80 - 144 277 99 78 177 128 X    
3 1251 60 - 49 - 135 - 80 - 144 301 99 78 177 128 1 N/A N/A N/A
4* 1227/1224 60 - 49 - 135 - 80 - 144 277/274 99 78 177 128 2 4 2 4
5* 1206 60 - 49 - 135 - 80 - 144 274 99 78 159 128   3   3
6 1188 60 42 49 - 135 - 80 - 144 274 99 - 177 128 X 1 X X
7 1173 60 - 49 - 135 - 80 - 144 301 99 - 177 128 2 N/A N/A N/A
8 1155 60 - 31 - 135 - 80 - 144 301 99 - 177 128 1 N/A N/A N/A
9* 1149/1146 60 - 49 - 135 - 80 - 144 277/274 99 - 177 128 2 8 1 2
10 1146+454 60 - 49 454 135 - 80 - 144 274 99 - 177 128   3   
11 1131 60 42 49 - - - 80 - 144 274 99 78 177 128   1   
12 1128 60 - 49 - 135 - 80 - 144 274 99 - 159 128   1   
13 1122 60 - 49 - - 51 80 - 144 274 99 78 159 128 N/A 1   
14 1122 60 - 31 - - 51 80 - 144 274 99 78 177 128 N/A 1   
15 1113 60 42 49 - - - 80 - 144 274 99 78 159 128   1   
16 1104 60 42 49 - - 51 80 - 144 274 99 - 177 128    1  
17*, B 1092/1089 60 - 49 - - - 80 - 144 277/274 99 78 177 128 1 8 4 2
18 1089+452 60 - 49 452 - - 80 - 144 274 99 78 177 128    1  
19* 1071 60 - 49 - - - 80 - 144 274 99 78 159 128   3   1
20 1071 60 - 31 - - - 80 - 144 274 99 78 177 128   1   
21 1062 60 - 49 - - 51 80 - 144 274 99 - 177 128 N/A 1   
22 1062+454 60 - 49 454 - 51 80 - 144 274 99 - 177 128 N/A 1   
23 1059 60 - 49 - - - 80 - 144 274 69 78 177 128   1   
24 1053 60 42 49 - - - 80 - 144 274 99 - 177 128     1
25 1044 60 - 49 - - 51 80 - 144 274 99 - 159 128   1   
26 1038 60 - 49 - - - 80 - 144 301 99 - 177 128 1 N/A N/A N/A
27 1026 60 - 31 - - 51 80 - 144 274 99 - 159 128 N/A 1   
28* 1014/1011 60 - 49 - - - 80 - 144 277/274 99 - 177 128 2 7 1 5
29* 1014/1011
+454/452
60 - 49 454/452 - - 80 - 144 277/274 99 - 177 128 1 1   
30* 996/993 60 - 49 - - - 80 - 144 277/274 99 - 159 128 1 1   1
31 965 60 - - - - - 80 - 144 277 99 - 177 128 1    
32 549 60 - 49 - 135 - - - - - -   177 128 1    
33* 414 60 - 49 - - - - - - - -   177 128 2   1  
34 378+454 60 - 31 454 - - - - - - - - 159 128 1    
35 ?      135 51 80         N/A X X X
  1. Alternatively spliced transcripts (AST) were analyzed in four different species of Drosophila: D. pseudoobscura (pse), D. persimilis (per), D. melanogaster (mel) and D. virilis (vir). The total number of sequenced transcripts is shown in brackets for each species. Two known ASTs of D. melanogaster (FlyBase.org) labeled with letters - A and B - are also included in the table. D. melanogaster exon 10 was longer by 3 nucleotides than the same exon in the other species. Thus, two sizes separated by a slash are presented for the ASTs shared between both species. Although we have not sequenced the entire ASTs 2 and 35, the splice choices specific for these ASTs were sampled for species designated by "X".
  2. * - AST was conserved between at least two distantly related Drosophila species.