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Table 1 Diversity of alternatively spliced transcripts of Bruno-3 identified in four different species of Drosophila

From: Large introns in relation to alternative splicing and gene evolution: a case study of Drosophila bruno-3

  

Size of translated exon (bp)

Number of sampled clones

AST

Size of ORF

2

3

4

4a

5

6

7

8

9

10

11

12

13

14

D. mel. (19)

D. pse. (51)

D. per. (11)

D. vir. (19)

1

1281

60

-

49

-

135

-

80

57

144

274

99

78

177

128

N/A

1

 

N/A

2, A

1269

60

42

49

-

135

-

80

-

144

277

99

78

177

128

X

   

3

1251

60

-

49

-

135

-

80

-

144

301

99

78

177

128

1

N/A

N/A

N/A

4*

1227/1224

60

-

49

-

135

-

80

-

144

277/274

99

78

177

128

2

4

2

4

5*

1206

60

-

49

-

135

-

80

-

144

274

99

78

159

128

 

3

 

3

6

1188

60

42

49

-

135

-

80

-

144

274

99

-

177

128

X

1

X

X

7

1173

60

-

49

-

135

-

80

-

144

301

99

-

177

128

2

N/A

N/A

N/A

8

1155

60

-

31

-

135

-

80

-

144

301

99

-

177

128

1

N/A

N/A

N/A

9*

1149/1146

60

-

49

-

135

-

80

-

144

277/274

99

-

177

128

2

8

1

2

10

1146+454

60

-

49

454

135

-

80

-

144

274

99

-

177

128

 

3

  

11

1131

60

42

49

-

-

-

80

-

144

274

99

78

177

128

 

1

  

12

1128

60

-

49

-

135

-

80

-

144

274

99

-

159

128

 

1

  

13

1122

60

-

49

-

-

51

80

-

144

274

99

78

159

128

N/A

1

  

14

1122

60

-

31

-

-

51

80

-

144

274

99

78

177

128

N/A

1

  

15

1113

60

42

49

-

-

-

80

-

144

274

99

78

159

128

 

1

  

16

1104

60

42

49

-

-

51

80

-

144

274

99

-

177

128

  

1

 

17*, B

1092/1089

60

-

49

-

-

-

80

-

144

277/274

99

78

177

128

1

8

4

2

18

1089+452

60

-

49

452

-

-

80

-

144

274

99

78

177

128

  

1

 

19*

1071

60

-

49

-

-

-

80

-

144

274

99

78

159

128

 

3

 

1

20

1071

60

-

31

-

-

-

80

-

144

274

99

78

177

128

 

1

  

21

1062

60

-

49

-

-

51

80

-

144

274

99

-

177

128

N/A

1

  

22

1062+454

60

-

49

454

-

51

80

-

144

274

99

-

177

128

N/A

1

  

23

1059

60

-

49

-

-

-

80

-

144

274

69

78

177

128

 

1

  

24

1053

60

42

49

-

-

-

80

-

144

274

99

-

177

128

   

1

25

1044

60

-

49

-

-

51

80

-

144

274

99

-

159

128

 

1

  

26

1038

60

-

49

-

-

-

80

-

144

301

99

-

177

128

1

N/A

N/A

N/A

27

1026

60

-

31

-

-

51

80

-

144

274

99

-

159

128

N/A

1

  

28*

1014/1011

60

-

49

-

-

-

80

-

144

277/274

99

-

177

128

2

7

1

5

29*

1014/1011

+454/452

60

-

49

454/452

-

-

80

-

144

277/274

99

-

177

128

1

1

  

30*

996/993

60

-

49

-

-

-

80

-

144

277/274

99

-

159

128

1

1

 

1

31

965

60

-

-

-

-

-

80

-

144

277

99

-

177

128

1

   

32

549

60

-

49

-

135

-

-

-

-

-

-

 

177

128

1

   

33*

414

60

-

49

-

-

-

-

-

-

-

-

 

177

128

2

 

1

 

34

378+454

60

-

31

454

-

-

-

-

-

-

-

-

159

128

1

   

35

?

    

135

51

80

       

N/A

X

X

X

  1. Alternatively spliced transcripts (AST) were analyzed in four different species of Drosophila: D. pseudoobscura (pse), D. persimilis (per), D. melanogaster (mel) and D. virilis (vir). The total number of sequenced transcripts is shown in brackets for each species. Two known ASTs of D. melanogaster (FlyBase.org) labeled with letters - A and B - are also included in the table. D. melanogaster exon 10 was longer by 3 nucleotides than the same exon in the other species. Thus, two sizes separated by a slash are presented for the ASTs shared between both species. Although we have not sequenced the entire ASTs 2 and 35, the splice choices specific for these ASTs were sampled for species designated by "X".
  2. * - AST was conserved between at least two distantly related Drosophila species.