Table 8 Estimated reagent and sequencing costs
| Reagent | Cost/well | Extended cost Plate 1 | Extended cost Plate 2 or 3 |
|---|---|---|---|
| Assay | $0.03 | ||
| Fast Start | $0.34 | ||
| dNTPs | $0.04 | ||
| 10× Buffer | $0.02 | ||
| Sub Total | $0.43 | ||
| X6/5* | $0.52 | ||
| X8/7# | $0.49 | ||
| Plastic ware | $0.06 | $0.06 | $0.06 |
| QIAxcel system | $0.25 | $0.25 | $0.25 |
| AmPure | $0.07 | $0.07 | $0.07 |
|
Total PCR reagents | $0.90/patient PCR | $0.87/patient PCR | |
| Big Dye | $0.90 | $1.80/patient PCRΘ | $1.80/patient PCR |
| Buffer | $0.06 | $0.12/patient PCR | $0.12/patient PCR |
| Plate | $0.01 | $0.02/patient PCR | $0.02/patient PCR |
| CleanSeq | $0.30 | $0.60/patient well | $0.60/patient PCR |
| Plate | $0.01 | $0.02/patient PCR | $0.02/patient PCR |
| Total per patient well | $3.46 | $3.43 | |
| Total per patient | $442.88 | $329.28 | |
| Add ~20% for waste | $532.00 | $395.00 |
