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Table 8 Estimated reagent and sequencing costs

From: Automated DNA mutation detection using universal conditions direct sequencing: application to ten muscular dystrophy genes

Reagent Cost/well Extended cost Plate 1 Extended cost Plate 2 or 3
Assay $0.03   
Fast Start $0.34   
dNTPs $0.04   
10× Buffer $0.02   
Sub Total $0.43   
X6/5*   $0.52  
X8/7#    $0.49
Plastic ware $0.06 $0.06 $0.06
QIAxcel system $0.25 $0.25 $0.25
AmPure $0.07 $0.07 $0.07
Total PCR
reagents
  $0.90/patient PCR $0.87/patient PCR
Big Dye $0.90 $1.80/patient PCRΘ $1.80/patient PCR
Buffer $0.06 $0.12/patient PCR $0.12/patient PCR
Plate $0.01 $0.02/patient PCR $0.02/patient PCR
CleanSeq $0.30 $0.60/patient well $0.60/patient PCR
Plate $0.01 $0.02/patient PCR $0.02/patient PCR
Total per patient well   $3.46 $3.43
Total per patient   $442.88 $329.28
Add ~20% for waste   $532.00 $395.00
  1. * for one negative control per 5 patients on plate 1; # one negative control per 7 patients on plates 2 or 3; Θ for both Forward and Reverse sequence/PCR.
  2. 20% waste value is a conservative estimate of waste established over many years and used by our clinical and research labs in calculating material waste. It includes remaining reagents in large cocktail batches and possible manual repeats.