TY - JOUR AU - Bennett, Richard R. AU - Schneider, Hal E. AU - Estrella, Elicia AU - Burgess, Stephanie AU - Cheng, Andrew S. AU - Barrett, Caitlin AU - Lip, Va AU - Lai, Poh San AU - Shen, Yiping AU - Wu, Bai-Lin AU - Darras, Basil T. AU - Beggs, Alan H. AU - Kunkel, Louis M. PY - 2009 DA - 2009/10/18 TI - Automated DNA mutation detection using universal conditions direct sequencing: application to ten muscular dystrophy genes JO - BMC Genetics SP - 66 VL - 10 IS - 1 AB - One of the most common and efficient methods for detecting mutations in genes is PCR amplification followed by direct sequencing. Until recently, the process of designing PCR assays has been to focus on individual assay parameters rather than concentrating on matching conditions for a set of assays. Primers for each individual assay were selected based on location and sequence concerns. The two primer sequences were then iteratively adjusted to make the individual assays work properly. This generally resulted in groups of assays with different annealing temperatures that required the use of multiple thermal cyclers or multiple passes in a single thermal cycler making diagnostic testing time-consuming, laborious and expensive. SN - 1471-2156 UR - https://doi.org/10.1186/1471-2156-10-66 DO - 10.1186/1471-2156-10-66 ID - Bennett2009 ER -