An amplicon containing rs9903378 and an amplicon containing rs9894946 were amplified in a multiplex PCR. A The melting curves of the different genotype combinations of the two SNPs show different profiles. In gray TT-CC, in blue TT-CT, in red GG-CC, in orange TG-CC and in purple TT-TT, respectively for rs9903378 and rs9894946. B The analysis was performed in the region of melting temperature of rs9903378 (75–80°C). in gray TT, in orange TG in red GG. C The analysis was performed in the region of melting temperature of rs9894946 (90–95°C). in gray CC, in blue CT in purple TT.