Simultaneous genotyping of rs858528, rs1641548, and rs1641549 using two unlabeled probes. A Samples that are heterozygous for rs858528, rs1641548, and rs1641549 have essentially indistinguishable melting curves (in red). B Genotyping using two unlabeled probes. Probe 1 targets the G allele of rs858528 and probe 2 targets the G allele of rs1641548 and the A allele of rs1641549. C Each distinct melting profile from panel B corresponds to a combination of genotypes of the three SNPs found in our population.