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Table 1 DNA Recovery from Two 3 mm Guthrie Card Punches

From: Whole genome microarray analysis, from neonatal blood cards

sample ID Yield (ng) Conc (ng/uL) Specimen Name Collection Date
1 261.7 26 530011 1995
2 203.37 20 529979 1995
3 162.33 16 529969 2003
4 312.9 31 529943 2003
5 380.43 38 529844 1994
6 212.16 21 529895 1994
7 319.9 32 520908 2001
8 188.41 19 529809 2001
9 246.88 65 MOD881 1993
10 244.55 65 MOD892 1993
11 225.47 23 529874 2003
12 131.31 13 529810 2003
13 391.96 50 MOD919 1998
14 367.67 48 MOD937 1998
15 161.23 16 529852 2000
16 264.59 26 529906 2000
17 255.67 57 MOD920 1995
18 226.43 65 MOD975 1995
19 268.02 69 MOD986 1995
20 234.94 53 MOD001 1995
21 350.78 35 530127 1995
22 339.53 34 530128 1991
23 224.1 22 529236 1992
24 274.61 27 529237 1997
overall average <260 ng> <26 ng/uL>   <1997>
Microarray samples <280 ng> <59 ng/uL>   <1995>
  1. DNA concentration was measured via PicoGreen analysis. Samples in red refer to those specimens used for microarray analysis, based on the requirement that their DNA concentration exceed 50 ng/uL to accommodate Illumina hybridization chemistry. Notice that the average DNA yield (in nanograms) for the 8 specimens used for microarray analysis and their average age did not deviate significantly from the average of all 24. However, due to volume variability in the final concentrated DNA sample, the mass concentration of the 8 specimens used for microarray analysis (59 ng/uL) was approximately twice that of the average over the entire set of twentyfour (26 ng/uL).