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Table 1 PCR primers used in this study

From: Mutations in genes involved in nonsense mediated decay ameliorate the phenotype of sel-12 mutants with amber stop mutations in Caenorhabditis elegans

Product

Sense primer

Antisense primer

enzyme

Rpl-12

GN1100 GTTGCGTCGGAGGAGAAGTCG

GN1101 GATGATGTCGTGTGGGTGTTGTC

 

ama-1

GN701 GTCACATGAAAGATGGCGATATAA

GN702 CATCATTGACTTTTGTGGAGAGT

 

Eft-2

GN739 ACTTGTTGGAGTCGATCAATACCT

GN740 GACTGGAGATACGGAGAACTTCAT

 

nhx-4

GN753 GGGTTGTACCATTAAGTTCGTCAT

GN754 TACTTGTCGTTCAGGTGAGTAAGC

 

sel-12-1

GN854 GGAGCATCTCACGTTATTCATCTA

GN855 CCCGGACAAAAGGAGTGTATAGTA

 

sel-12-3

GN876 TGGACTGGGTAACTATGGAGTTCT

GN877 GATAAAGACCAGAGCCATTAGTGC

 

sel-12-5

GN880 AGAGAGAGGTGTGAAACTTGGTCT

GN881 AGTGAAGCAGAGACCGATAAGAAT

 

sel-12-6

GN882 CTTCCACAAGGAGACAACAGG

GN883 ATAACGTGAGATGCTCCGTATTTC

 

Y22D7AL[4]

GN37 CTTACGCCAAGGACGTCAAG

GN38 GGTATTTGTCCTTGAGGTCG

DraI

pkP3086

GN553 TTGAGAAGAATGAGCAGAGCGG

GN554 AACTGATGGCCTAGAAATCCAAGGAGTTCG

DdeI

Y71H2B[2]

GN39 AAGAAGAATTAGGCGATGCGG

GN40 CTGAAAACTTGGAAAATCGGTG

DraI

C48B6[1]

GN124 GTCAAGGACCATTGTTACGAG

GN125 GGGCAGTTATTAGTTCGTGAG

DraI

byP4

GN1008 GAAATTTCAGTGCCACTTCC

GN1009 CTAGGCAGTACATAAATGCG

KpnI

smg-1(by146)

GN1222 CTTCATCTTGATGAACGAGTCATGC

GN1213 AGAAGTAGAACGGACTATGACACG

 

smg-1(by146)

GN1222 CTTCATCTTGATGAACGAGTCATGC

GN1264 CATGATCTGCGAATGCTAGACG

 
  1. Primers used for quantitative PCR, the rpl-12 test and Single Nucleotide Polymorphism (SNP) mapping are noted. For SNPs, the restriction enzyme used to detect the polymorphism in indicated in the last column.