The effect of spr-2 on sel-12 and rpl-12 transcripts levels. A) The expression of sel-12 transcripts during development in: N2, LA242 sel-12(ty11), LA259 spr-2(ar199); sel-12(ty11) and smg-2(e2008); sel-12(ty11) strains. The relative mRNA levels were determined by qRT-PCR with primer pairs for amplicon sel-12-6 (Table 1). The X axis represents time in hours after feeding synchronous starved L1s as described in the Methods section. (-) is asynchronous eggs. B) A scheme of the alternative splicing of rpl-12. Coding exons are shown in dark blue, the alternative 3' extension of exon 2 is shown in light blue with a bar and a * to indicate a stop codon. The locations of the primers used to detect rpl-12 transcripts are noted. C)rpl-12 mRNA splicing assay for Smg activity. RNA was prepared from synchronous L1s. Pictures are from Ethidium bromide-stained 2% agarose gel. The sizes of DNA fragments from the loading ladder are shown at the left. The gN2 lane is a control with N2 genomic DNA.