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Table 5 Comparison of Chromosome 4 and 11 ENU Balancer Screens

From: A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines

Parameter

Chr 4

(117–281)

Chr 4

(281–51)

Chr 11

(Trp53-Wnt3)

Interval Breakpoints

96.4–129.8 Mb

129.8–154.4 Mb

69.4–103.7 Mb

Interval size

33 Mb

25 Mb

34 Mb

Number of annotated genes

470

461

905

Pedigrees screened

530

551

785

Lethal lines

11

19

59

Other viable phenotypes

0

4

32

Total mutations

11

23

91

Mutations/Mb

0.33

0.92

2.68

Homogeneity of samplesa

3.913, p < 0.05

22.8, p < 0.001

37.2, p < 0.001

Frequency of mutations mapping outside of the balancer region

1 in 5.4

1 in 4.8

1 in 4.2

Literature Reference

This report

Hentges et al. 2006 [4]

Kile et al. 2003 [5]

  1. In all cases, the phenotype screen included a coat color screen for all lethals in the balancer region. It also included a complete blood count for all blood cell parameters, a screen for neutral cholesterols using tandem mass spectrometry, a urine screen for albumin and glucose, and a general visual assessment of skin, coat, eyes, fur, movement and balance, as well as morphology on all viable mice, which would carry mutations segregating to the balancer region or elsewhere in the genome.
  2. a A Chi square test for homogeneity of samples in a binomial distribution with one degree of freedom. The values represent the significance from the numbers of mutations isolated in the next column, with the exception that the number in column c is the difference from column a.