Figure 2

Chromosome 4 (117–281) Lethal Mutants. Mutant embryos are pictured on the right and littermate controls are pictured on the left. All of the images of mutants and control from the same line were taken at the same magnification except D in which the mutant was photographed at a higher magnification in order to better visualize the allantois. A. Hematoxylin and Eosin stained sections of a presumed l4Jus29 homozygous mutant (right) and control littermate (left) are pictured at E7.5. The mutants die prior to gastrulation. Because of lack of embryonic tissue, these mutants were not genotyped but were identified by abnormal histology. B. l4Jus26 at E8.5. Mutants are smaller than control littermates and exhibit developmental delay. C. l4Jus25 at E10.5. Mutants display cardiovascular defects and are smaller than control littermates. Pericardial edema is evident in the mutant and is indicated with a white arrow. D. l4Jus28 at E9.5. Mutant embryos have not turned and the allantois grows but does not fuse with the chorion, shown with a white arrow. E. l4Jus30 at E11.5. Mutant embryos are smaller than control littermates and exhibit growth defects, as well as abnormal telencephalon development.