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Figure 1 | BMC Genetics

Figure 1

From: A mouse chromosome 4 balancer ENU-mutagenesis screen isolates eleven lethal lines

Figure 1

Chromosome 4 (117–281) Balancer Screen. A. The balancer regions used in this screen (red) and in a previously published screen (dark gray) are shown. Genotyping microsatellite markers are shown in red. Base pairs refer to Ensembl v51. B. The mating scheme used for the chromosome 4 (117–281) balancer screen is shown. Red or "m" indicates a new ENU-induced lesion and black indicates the balancer chromosome. Males are depicted with squares and females are depicted with circles. Diamonds represent either sex. C. The mouse chromosome 4 balancer is depicted with a red line. Microsyntenies, representing 50–800 bp fragments and averaging 100–200 bp, were blasted and aligned to full-length human chromosome 1, depicted with a blue line. Light blue lines connect each hit of microsynteny conservation between the two chromosomes. Two large areas of linkage conservation are evident on the human chromosome. D-G. Homozygous mutants, heterozygous balancer animals, and homozygous balancer animals are all easily distinguished by coat color and the presence or absence of eye pigment at several different stages. A homozygous lethal embryo (no eye pigment) is pictured at E15.5 (D, right) next to a control littermate (D, left). An albino mutant pup lacking eye pigment is easily distinguished from control littermates at P3 (E, far right). Homozygous mutants (white, no eye pigment), heterozygous balancer animals (light brown), and homozygous balancer animals (dark brown) are shown at both the P11 (F) and adult stages (G) and can easily be differentiated by coat color.

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