Microscopic observation of hyphae expressing the S7GFP protein. A-F, are experimental observations with strains carrying the su12-GFP chimaeric gene. A, C and E visualise GFP fluorescence and B, D and F visualise DAPI straining. The hyphae in A and B is from the growing edge; The hyphae in C and D is taken 1 centimetre away from the growing edge corresponding to about 1 day of stationary phase. The hyphae in E and F is taken 5 centimetres away from the growing edge corresponding to about 1 week of stationary phase. The picture in E was taken with a pose time twice as long as the other pictures. G (GFP fluorescence) and H (DAPI staining) are control observations of GFP alone expressed from the GPD promoter; the hyphae is taken 1 centimetre away from the growing edge as in C and D. I (GFP fluorescence), J (DAPI staining) and K (superposition of I and J) show the sub-nuclear localisation of the foci in two adjacent nuclei. Clearly, the GFP foci are located in a depression of the DAPI staining, where the nucleolus is supposed to be located.